Specific Detection of the Gene for the Extracellular Neutral Protease of Bacillus cereus by PCR and Blot Hybridization-Reference-Cited by-同舟云学术

Specific Detection of the Gene for the Extracellular Neutral Protease of Bacillus cereus by PCR and Blot Hybridization

Published:1999-07 Issue:7 Volume:65 Page:3226-3228
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Bach H.-J.¹,Errampalli D.²,Leung K. T.²,Lee H.²,Hartmann A.¹,Trevors J. T.²,Munch J. C.¹

Affiliation:

1. Institute for Soil Ecology, GSF-National Research Center for Environment and Health, 85764 Neuherberg, Germany,1 and

2. Department of Environmental Biology, University of Guelph, Guelph, Ontario, Canada N1G 2W12

Abstract

ABSTRACT A pair of primers and a gene probe for the amplification and detection of the Bacillus cereus neutral protease gene (NPRC) were developed. Specificity for the npr genes of the B. cereus group members B. cereus , B. mycoides , and B. thuringiensis was shown. Restriction polymorphism patterns of the PCR products confirmed the presence of the NPRC gene in all three species.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.65.7.3226-3228.1999

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