Affiliation:
1. Department of Food Engineering, Yonsei University, Seoul, Korea.
Abstract
One of the cellulase genes from alkalophilic Bacillus sp. strain N-4 was cloned in pBR322. A recombinant plasmid, pYBC107, expressing carboxymethyl cellulase (CMCase) was isolated, and the size of the cloned HindIII fragment was found to be 5.5 kilobases. The restriction map of pYBC107 showed a different pattern from those of pNKI and pNKII (N. Sashihara, T. Kudo, and K. Horikoshi, J. Bacteriol. 158:503-506, 1984). When the HindIII fragment from pYBC107 was subcloned into pYEJ001, there was a 3.8-fold increase in CMCase activity over that observed with pYBC107. Plasmid pYBC108 constructed by treatment of pYBC107 with HindIII and EcoRI expressed the CMCase activity, although to a limited extent. To verify the originality of cloned pYBC107 from Bacillus sp., we analyzed the restriction digest by Southern blotting.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference21 articles.
1. Molecular cloning of a Bacillus subtilis xylanase gene in Escherichia coli;Bernier R.;Gene,1983
2. Construction and characterization of new cloning vehicles. II. A multipurpose cloning system;Bolivar F.;Gene,1977
3. Molecular cloning of a Bacillus subtilis xylanase gene in Escherichia coli;Cantwell B. A.;Gene,1983
4. Cloning and expression of a Thermomonospora YX endocellulase gene in E. coli;Collmer A.;Bio/Technology,1983
5. Characterization of two cel (cellulose degradation) genes of Clostridium thermocellum coding for endoglucanases;Cornet P.;Bio/ Technology,1983
Cited by
13 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献