Wood Degradation by White Rot Fungi: Cytochemical Studies Using Lignin Peroxidase-Immunoglobulin-Gold Complexes

Author:

Garcia Susana1,Latge Jean Paul1,Prevost Marie Christine1,Leisola Matti1

Affiliation:

1. Unit� de Mycologie and Station Centrale de Microscopie Electronique, 2 Institut Pasteur, 75724 Paris Cedex 15, France, and Institut f�r Biotechnologie, Eidgenosische Technische Holzschule-H�nggerberg, CH-8093 Zurich, Switzerland3

Abstract

Using an anti-lignin peroxidase antiserum-protein A-gold complex, we found lignin peroxidase mainly intracellularly in several white rot fungi colonizing sawdust under laboratory conditions. This enzyme was also present in fungi found in naturally decayed wood. However, in all cases, lignin peroxidase was located mainly inside the fungal cells. Labeled lignin peroxidase did not bind to the lignocellulosic samples tested, with the exception of poplar milled-wood lignin. These results are discussed in relation to the role of lignin peroxidase during wood degradation.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference28 articles.

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3. Resin development for electron microscopy and an analysis of embedding at low temperature;Carlemalm E.;J. Microsc. (Oxford),1982

4. A cytochemical investigation of the lateral walls of Dianthus vessels. Differentiation and PITmembrane formation;Catesson A. M.;Int. Assoc. Wood Anat. Bull.,1983

5. Mycelium binding and depolymerization of synthetic 14C-labeled lignin during decomposition by Phanerochaete chrysosporium;Chua M. G. S.;Holzforschung,1983

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