Affiliation:
1. Department of Plant Pathology, Oklahoma State University, Stillwater, Oklahoma 74078-0285
Abstract
A rapid filter paper dot-immunobinding assay was adapted to detect the wall-less mollicute
Spiroplasma citri
in medium, plants, or insects. Filter paper spotted with sample was incubated first in dilute antiserum, then in protein A-peroxidase, and finally in a substrate of 4-chloro-1-naphthol plus hydrogen peroxide. The detection limit averaged 2.3 � 10
10
CFU/ml in cultures, and
S. citri
was detected in single infected leafhoppers. This assay was less sensitive but more rapid and economical than an enzyme-linked immunosorbent assay.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
13 articles.
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