Affiliation:
1. Department of Neuropharmacology, Scripps Research Institute, La Jolla, California.
Abstract
Expression of alpha interferon (IFN-alpha)-, IFN-beta-, and IFN-alpha/beta-induced genes was monitored during the development of lymphocytic choriomeningitis (LCM) to assess whether a restricted influence of these antiviral cytokines could be found in the central nervous system (CNS). High levels of IFN-alpha (83 +/- 42 U/ml) were present in the blood of LCM virus-infected mice 3 days postinfection, whereas IFN-beta was not detected (< 1.0 U/ml) at any time point. Spleens contained high levels of IFN-alpha and IFN-beta mRNAs at days 1 and 3 postinfection, whereas no IFN-alpha mRNA and only low levels of IFN-beta mRNA were detected in brains. In situ hybridization showed IFN-alpha mRNA-expressing cells in the marginal zones of the spleen and in the subcapsular sinus and outer cortex of cervical lymph nodes. The expression of 2',5'-oligoadenylate synthetase (2',5'-OAS) mRNA followed the expression of IFN-beta mRNA in the brain, whereas 2',5'-OAS mRNA in the periphery was associated with systemic IFN-alpha. The localization of IFN-alpha-expressing cells in the spleen and lymph nodes in proximity to T- and B-cell compartments is consistent with a role for these cytokines in immune regulation. Furthermore, the absence of IFN-alpha and the relatively low level and delayed expression of IFN-beta in the brain suggest that the CNS is an especially vulnerable organ for virus replication. With certain strains of LCM virus, the absence of early antiviral IFN-alpha/beta activity and preferential virus growth in the brain might lead to targeted T-cell inflammation of the CNS, resulting in death of the animal.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference57 articles.
1. Interferon-gamma regulation of major histocompatibility class I gene expression in rat cells containing the adenovirus 12E1A oncogene;Ackrill A. M.;Virology,1990
2. la.Arturssan K. M. Lindersson A. Scheynius and G. V. Alm. Interferon-a production and tissue localization of interferon-a/ B producing cells after intradermal administration of Aujeszky's disease virus-infected cells in pigs. Submitted for publication.
3. A simple, rapid method for the purification of poly A;Badley J. E.;RNA. BioTechniques,1988
4. Requirement for HLA-DR+ accessory cells in natural killing of cytomegalovirus-infected fibroblasts;Bandyopadhyay S.;J. Exp. Med.,1986
5. The virology and immunology of Iymphocytic choriomeningitis virus infection;Buchmeier M. J.;Adv. Immunol.,1980
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