Affiliation:
1. Department of Microbiology and Molecular Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Abstract
In this study, we investigated the interaction of reovirus particles with cell membranes by using a 51Cr release assay. We confirmed prior observations (J. Borsa, B. D. Morash, M. D. Sargent, T. P. Copps, P. A. Lievaart, and J. G. Szekely, J. Gen. Virol. 45:161-170, 1979) that intermediate subviral particles (ISVPs) of reovirus type 3 strain Abney (T3A) induced the release of 51Cr from preloaded L cells and showed that the intact virion and core forms did not. Reovirus type 1 strain Lang (T1L) ISVPs were found to be less efficient at 51Cr release than T3A ISVPs. Reassortants between these strains indicated that the 51Cr release phenotype segregates with the M2 gene segment. Biochemical studies indicated that the ISVPs' acquisition of the capacity to induce 51Cr release followed the cleavage of the viral M2 gene product mu 1/mu 1C to fragments delta and phi during virion conversion to ISVP but did not directly correlate with this cleavage. These studies suggest that the reovirus M2 gene product (in its cleaved form) plays a role in interacting with cell membranes.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference31 articles.
1. New intermediate subviral particles in the in vitro uncoating of reovirus virions by chymotrypsin;Borsa J.;J. Virol.,1973
2. Two modes of entry of reovirus particles into L cells;Borsa J.;J. Gen. Virol.,1979
3. Reovirus: evidence for a second step in the intracellular uncoating and transcriptase activation process;Borsa J.;Virology,1981
4. Brown E. G. M. L. Nibert and B. N. Fields. 1983. The L2 gene of reovirus serotype 3 controls the capacity to interfere accumulate deletions and establish persistent infection p. 275-287. In R. W. Compans and D. H. L. Bishop (ed.) Double stranded RNA viruses. Elsevier Science Publishing Inc. New York.
5. Ammonium chloride prevents Iytic growth of reovirus and helps to establish persistent infection in mouse L cells;Canning W. M.;Science,1983
Cited by
89 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献