Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase I. Optimal Conditions for In Vitro Activity of the Ribonucleic Acid-Dependent Ribonucleic Acid Polymerase-Reference-Cited by-同舟云学术

Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase I. Optimal Conditions for In Vitro Activity of the Ribonucleic Acid-Dependent Ribonucleic Acid Polymerase

Published:1971-07 Issue:1 Volume:8 Page:66-73
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Bishop David H. L.¹²,Obijeski John F.¹²,Simpson Robert W.¹²

Affiliation:

1. Institute of Cancer Research, Columbia University New York, New York 10032

2. Institute of Microbiology, Rutgers University, New Brunswick, New Jersey 08903

Abstract

In vitro reaction conditions have been determined for the maximal synthesis of product ribonucleic acid by the influenza (WSN) virion ribonucleic acid polymerase. The reaction requires the presence of all four triphosphates, Mg 2+ and Mn 2+ ions, monovalent cations, nonionic detergent, and ribonucleoside triphosphates at concentrations above certain threshold values. The optimum p H for the reaction is around 8.0 to 8.2 and the kinetics of product synthesis are linear through at least 6 hr when incubated at 31 to 33 C.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.8.1.66-73.1971

Reference15 articles.

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3. Myxovirus ribonucleic acids. Annu;Blair C. D.;Rev. Microbiol.,1970

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5. The ribonucleic acids of the infective and interfering components of vesicular stomatitis virus;Brown F.;J. Gen. Virol.,1967

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