Engineering the Soil Bacterium Pseudomonas putida for Arsenic Methylation

Author:

Chen Jian12,Qin Jie1,Zhu Yong-Guan23,de Lorenzo V�ctor4,Rosen Barry P.1

Affiliation:

1. Department of Cellular Biology and Pharmacology, Florida International University, Herbert Wertheim College of Medicine, Miami, Florida, USA

2. State Key Lab of Regional and Urban Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, China

3. Key Lab of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, People's Republic of China

4. Centro Nacional de Biotecnolog�a-CSIC, Campus UAM-Cantoblanco, Madrid, Spain

Abstract

Accumulation of arsenic has potential health risks through consumption of food. Here, we inserted the arsenite [As(III)] S -adenosylmethionine methyltransferase (ArsM) gene into the chromosome of Pseudomonas putida KT2440. Recombinant bacteria methylate inorganic arsenic into less toxic organoarsenicals. This has the potential for bioremediation of environmental arsenic and reducing arsenic contamination in food.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Reference20 articles.

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4. Market basket survey shows elevated levels of As in South Central U.S. processed rice compared to California: consequences for human dietary exposure;Williams PN;Environ. Sci. Technol.,2007

5. Biotransformation of arsenic by a Yellowstone thermoacidophilic eukaryotic alga;Qin J;Proc. Natl. Acad. Sci. U. S. A.,2009

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