Affiliation:
1. Departments of Chemistry and Microbiology, and the Stella Duncan Memorial Research Institute, University of Montana, Missoula, Montana 59801
Abstract
Ribosomal subunits from
Mycobacterium smegmatis
were analyzed by using sedimentation velocity, sedimentation equilibrium, and acrylamide gel electrophoresis experiments. These s
0.w
values for the subunits are 48.7
S
and 28.1
S
. The molecular weight of the 49
S
subunit is about 1.65 × 10
6
, and that of the 28
S
subunit is 7.8 × 10
5
. Both subunits contain about 37% protein and 63% ribonucleic acid. A protein-deficient particle having an s
0.w
value of 15.7
S
contains about 11% protein and 89% ribonucleic acid. Skin tests showed all subunits and proteins to be active as agents in provoking delayed hypersensitivity, but the 16
S
protein-deficient particle, as well as the proteins derived from it, was more specific than the subunits themselves.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Cited by
17 articles.
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