Affiliation:
1. Department of Microbiology, University of Illinois, Urbana, Illinois 61801
Abstract
Aerobic and anaerobic cultural techniques and histological methods were used in a study of the effects of environmental and dietary stress on the indigenous microbiota of the gastrointestinal tract of mice. Mice previously inoculated with
Salmonella typhimurium
were examined in a similar manner. Three strains of mice (CD-1, Ha/ICr, and C57BL) were used. Control animals previously inoculated with
S. typhimurium
had low population levels of
Salmonella
bacteria in the small and large bowel. Mice previously inoculated with
Salmonella
and then deprived of food, water, and bedding for 48 h harbored high population levels of these bacteria in their small and large bowels. Coliforms increased in numbers in the large bowel of stressed mice inoculated with
Salmonella
and in the jejunum-ileum and cecum of stressed mice not previously inoculated with
Salmonella
. Control mice had high population levels of lactobacilli inhabiting the keratinized squamous epithelium of the stomach. Stressed mice showed dramatic reductions in these populations of lactobacilli. Populations of fusiform-shaped bacteria associated with the mucosal epithelium of the cecum and colon in control mice were reduced in stressed mice as determined by microscope examination of histological sections. Total anaerobic counts were similar, however, in both stressed and control animals. Environmental and dietary stress markedly alter the gastrointestinal microbiota in mice. Therefore, such stressful conditions profoundly affect the factors that regulate the localization and population levels of microorganisms in the stomach and intestines.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference27 articles.
1. Effect of the normal microbial flora on the resistance of the small intestine to infection;Abrams G. D.;J. Bacteriol.,1966
2. Effect of the normal microbial flora on gastrointestinal motility;Abrams G. D.;Proc. Soc. Exp. Biol. Med.,1967
3. The occurrence of Escherichia coli somatic antibody in pig serum, colostrum and milk and an investigation of its possible significance in immunity;Arbuckle J. B. R.;Brit. Vet. J.,1968
4. Gastric secretion of mucous related to adrenocortical activity. A histochemical study in the rat;Arnthorsson G.;Scand. J. Gastroenterol.,1971
5. Immunological responses and microorganisms indigenous to the gastrointestinal tract;Berg R. D.;Amer. J. Clin. Nutr.,1972
Cited by
306 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献