Infectious Rous Sarcoma Virus and Reticuloendotheliosis Virus DNAs

Author:

Cooper Geoffrey M.1,Temin Howard M.1

Affiliation:

1. McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706

Abstract

An efficient and quantitative assay for infectious Rous sarcoma virus and reticuloendotheliosis virus DNAs is described. The specific infectivities of viral DNA corresponded to one infectious unit per 10 5 to 10 6 viral DNA molecules. Infection with viral DNA followed one-hit kinetics. The minimal size of infectious Rous sarcoma virus DNA was approximately 6 million daltons, whereas the minimal size of infectious reticuloendotheliosis virus DNA was larger, 10 to 20 million daltons.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

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