Affiliation:
1. Department of Biochemistry, Stanford University Medical School, Stanford, California 94305
2. Université de Genève, Départment de Biologie Moléculaire, Sciences II, CH-1211-Genève 4, Switzerland
Abstract
A λ
dvgal
plasmid carrying genes for controlled plasmid replication from phage λ and the bacterial
gal
operon was isolated as a deletion mutant of phage λ
gal
q4, which carries the
gal
operon between λ genes
P
and
Q
. The plasmid DNA was found in cell extracts as covalently closed circular molecules. The plasmid was characterized by using genetic crosses, digestion with the specific endonuclease
Eco
RI, sucrose gradient centrifugation, and electron microscopy. In one clone analyzed, the plasmid was a complete dimer (
O
λ
P
λ
gal
O
λ
P
λ
gal
); in a subclone derived from it, the plasmid was a partial dimer with only one copy of
gal
(
O
λ
P
λ
O
λ
P
λ
gal
). The partial dimer may be a recombination product of the complete dimer, since test crosses show that the
gal
and λ sequences in the plasmid can be separated by recombination. Analyses of the
Eco
RI digests of plasmid DNAs indicated one cleavage site per λ gene sequence and none in the
gal
operon. A λ
dvgal
monomer was approximately 6.7 × 10
6
daltons and the λ gene and
gal
components were 3.9 × 10
6
and 2.8 × 10
6
daltons, respectively. The λ
dvgal
plasmid can be introduced into a new bacterial host by transfection at an efficiency of 10
−6
per DNA molecule.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference21 articles.
1. Characterization of polypeptides made in vitro from bacteriophage lambda DNA;Allet B.;J. Mol. Biol.,1973
2. Berg D. 1971. Regulation in phage with duplications of the immunity region p. 667-678. In A. D. Hershey (ed.) The bacteriophage lambda. Cold Spring Harbor Laboratory. Cold Spring Harbor N.Y.
3. Genetic evidence for two types of gene arrangements in new Xdv plasmid mutants;Berg D.;J. Mol. Biol.,1974
4. Electron microscope study of the structure of Adv DNAs;Chow L.;J. Mol. Biol.,1974
5. Recombination deficient mutants of E. coli and other bacteria. Annu;Clark A.;Rev. Genet,1973
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