Use of Paired Serum Samples for Serodiagnosis of Typhoid Fever

Author:

House Deborah12,Chinh Nguyen T.3,Diep To S.4,Parry Christopher M.25,Wain John26,Dougan Gordon6,White Nicholas J.72,Hien Tran Tinh4,Farrar Jeremy J.72

Affiliation:

1. Centre for Molecular Microbiology and Infection, Department of Biological Sciences, Imperial College London, SW7 2AZ, United Kingdom

2. Wellcome Trust Clinical Research Unit, Centre for Tropical Diseases, Ho Chi Minh City, Vietnam

3. Department of Infectious Diseases, Faculty of Medicine, University of Medicine and Pharmacy, Ho Chi Minh City, Vietnam

4. Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam

5. Department of Medical Microbiology, University of Liverpool, Liverpool, L69 3GA, United Kingdom

6. The Wellcome Trust Sanger Institute, The Wellcome Trust Genome Campus, Hinxton, Cambridge, CB10 1SA, United Kingdom

7. Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, John Radcliffe Hospital, University of Oxford, Oxford, OX3 9DU, United Kingdom

Abstract

ABSTRACT Using an enzyme-linked immunosorbent assay we demonstrate that, in adult patients with typhoid fever, the sensitivity of a serological test based on the detection of anti-lipopolysaccharide immunoglobulin G is increased when used with paired serum samples taken 1 week apart.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference15 articles.

1. Anonymous. 1978. Typhoid and its serology. Br. Med. J.1:389-390.

2. Improved Serodiagnosis of Salmonella Enteric Fevers by an Enzyme-Linked Immunosorbent Assay

3. Brodie, J. 1977. Antibodies in the Aberdeen typhoid outbreak of 1964. I. The Widal reaction. J. Hyg. Camb.79:161-179.

4. Cherian, T., G. Sridharan, V. Mohandas, and T. Jacob John. 1990. Prevalence of Salmonella Typhi O and H antibodies in the serum of infants and pre-school children. Indian Pediatr.27:293-294.

5. A Randomized Controlled Comparison of Azithromycin and Ofloxacin for Treatment of Multidrug-Resistant or Nalidixic Acid-Resistant Enteric Fever

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