Affiliation:
1. Institut für Molekulare Mikrobiologie und Biotechnologie, Westfälische Wilhelms-Universität Münster, D-48149 Münster, Germany
Abstract
ABSTRACT
Gene transfer systems for
Gordonia polyisoprenivorans
strains VH2 and Y2K based on electroporation and conjugation, respectively, were established. Several parameters were optimized, resulting in transformation efficiencies of >4 × 10
5
CFU/μg of plasmid DNA. In contrast to most previously described electroporation protocols, the highest efficiencies were obtained by applying a heat shock after the intrinsic electroporation. Under these conditions, transfer and autonomous replication of plasmid pNC9503 was also demonstrated to proceed in
G. alkanivorans
DSM44187,
G. nitida
DSM44499
T
,
G. rubropertincta
DSM43197
T
,
G. rubropertincta
DSM46038, and
G. terrae
DSM43249
T
. Conjugational plasmid DNA transfer to
G. polyisoprenivorans
resulted in transfer frequencies of up to 5 × 10
−6
of the recipient cells. Recombinant strains capable of polyhydroxyalkanoate synthesis from alkanes were constructed.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Cited by
36 articles.
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