Development of a Sensitive Gene Expression Reporter System and an Inducible Promoter-Repressor System for Clostridium acetobutylicum-Reference-Cited by-同舟云学术

Development of a Sensitive Gene Expression Reporter System and an Inducible Promoter-Repressor System for Clostridium acetobutylicum

Published:2003-08 Issue:8 Volume:69 Page:4985-4988
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Girbal Laurence¹,Mortier-Barrière Isabelle²,Raynaud Frédéric¹,Rouanet Céline¹,Croux Christian¹,Soucaille Philippe¹

Affiliation:

1. Centre de Bioingénierie Gilbert Durand, Laboratoire de Biotechnologie-Bioprocédés, UMR CNRS 5504, UMR INRA 792, INSA

2. CRT/CRITT-Bioindustries, INSA, DGBA, 31077 Toulouse cedex 4, France

Abstract

ABSTRACT A sensitive gene expression reporter system was developed for Clostridium acetobutylicum ATCC 824 by using a customized gusA expression cassette. In discontinuous cultures, time course profiles of β-glucuronidase specific activity reflected adequately in vivo dynamic up- and down-regulation of acidogenesis- and/or solventogenesis-associated promoter expression in C. acetobutylicum . Furthermore, a new inducible gene expression system was developed in C. acetobutylicum , based on the Staphylococcus xylosus xylose operon promoter-repressor regulatory system.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.69.8.4985-4988.2003

Reference24 articles.

1. Dürre, P., M. Böhringer, S. Nakotte, S. Schaffer, K. Thormann, and B. Zickner. 2002. Transcriptional regulation of solventogenesis in Clostridium acetobutylicum.J. Mol. Microbiol. Biotechnol.4:295-300.

2. Transmembrane pH of Clostridium acetobutylicum is inverted (more acidic inside) when the in vivo activity of hydrogenase is decreased

3. Molecular characterization and transcriptional analysis of the putative hydrogenase gene of Clostridium acetobutylicum ATCC 824

4. Northern, Morphological, and Fermentation Analysis of spo0A Inactivation and Overexpression in Clostridium acetobutylicum ATCC 824

5. Jefferson, R. A. 1989. The GUS reporter gene system. Nature342:837-838.

Cited by 60 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. A toolbox for genetic manipulation in intestinal Clostridium symbiosum;Synthetic and Systems Biotechnology;2024-03

2. Improvement of the Genome Editing Tools Based on 5FC/5FU Counter Selection in Clostridium acetobutylicum;Microorganisms;2023-11-03

3. Refined control of CRISPR-Cas9 gene editing in Clostridium sporogenes: the creation of recombinant strains for therapeutic applications;Frontiers in Immunology;2023-10-05

4. Engineering the Metabolic Profile of Clostridium cellulolyticum with Genomic DNA Libraries;Fermentation;2023-06-27

5. Heterologous Gene Regulation in Clostridia: Rationally Designed Gene Regulation for Industrial and Medical Applications;ACS Synthetic Biology;2022-10-20