Affiliation:
1. Graduate School of Biological Sciences, Nara Institute of Science and Technology, 8916-5 Takayama, Ikoma, Nara 630-0192, Japan
Abstract
ABSTRACT
Rhodococcus erythropolis
N9T-4, which was isolated from crude oil, showed extremely oligotrophic growth and formed its colonies on a minimal salt medium solidified using agar or silica gel without any additional carbon source. N9T-4 did not grow under CO
2
-limiting conditions but could grow on a medium containing NaHCO
3
under the same conditions, suggesting that the oligotrophic growth of N9T-4 depends on CO
2
. Proteomic analysis of N9T-4 revealed that two proteins, with molecular masses of 45 and 55 kDa, were highly induced under the oligotrophic conditions. The primary structures of these proteins exhibited striking similarities to those of methanol:
N
,
N
′-dimethyl-4-nitrosoaniline oxidoreductase and an aldehyde dehydrogenase from
Rhodococcus
sp. These enzyme activities were three times higher under oligotrophic conditions than under
n
-tetradecane-containing heterotrophic conditions, and gene disruption for the aldehyde dehydrogenase caused a lack of growth on the minimal salt medium. Furthermore, 3-hexulose 6-phosphate synthase and phospho-3-hexuloisomerase activities, which are key enzymes in the ribulose monophosphate pathway in methylotrophic bacteria, were detected specifically in the cell extract of oligotrophically grown N9T-4. These results suggest that CO
2
fixation involves methanol (formaldehyde) metabolism in the oligotrophic growth of
R. erythropolis
N9T-4.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
38 articles.
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