Affiliation:
1. Department of Fermentation Technology, Osaka University, Suita-shi, Osaka, Japan
Abstract
Saccharomyces cerevisiae
strain H-42 seems to have two kinds of acid phosphatase: one which is constitutive and one which is repressible by inorganic phosphate. The constitutive enzyme was significantly unstable to heat inactivation, and its
K
m
of 9.1 × 10
−4
m
for
p
-nitrophenylphosphate was higher than that of the repressible enzyme (2.4 × 10
−4
m
). The constitutive and the repressible acid phosphatases are specified by the
phoC
gene and by the
phoB
,
phoD
, or
phoE
gene, respectively. Results of tetrad analysis suggested that the
phoC
and
phoE
genes are linked to the
lys2
locus on chromosome II. Since both repressible acid and alkaline phosphatases were affected simultaneously in the
phoR, phoD
, and
phoS
mutants, it was concluded that these enzymes were under the same regulatory mechanism or that they shared a common polypeptide. The
phoR
mutant produced acid phosphatase constitutively, and the
phoR
mutant allele was recessive to its wild-type counterpart. The
phoS
mutant showed a phenotype similar to that of a mutant defective in one of the
phoB, phoD
, or
phoE
genes. However, the results of genetic analysis of the
phoS
mutant clearly indicated that the
phoS
gene is not a structural gene for either of the repressible acid and alkaline phosphatases, but is a kind of regulatory gene. According to the proposed model, the
phoS
gene controls the expression of the
phoR
gene, and inorganic phosphate would act primarily as an inducer for the formation of the
phoR
product which represses phosphatase synthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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