Accuracy of Enzyme-Linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis-Reference-Cited by-同舟云学术

Accuracy of Enzyme-Linked Immunosorbent Assay Using Crude and Purified Antigens for Serodiagnosis of Melioidosis

Published:2007-01 Issue:1 Volume:14 Page:110-113
ISSN:1556-6811
Container-title:Clinical and Vaccine Immunology
language:en
Short-container-title:Clin Vaccine Immunol

Author:

Chantratita Narisara¹²³,Wuthiekanun Vanaporn¹²³,Thanwisai Aunchalee¹²³,Limmathurotsakul Direk¹²³,Cheng Allen C.¹²³,Chierakul Wirongrong¹²³,Day Nicholas P. J.¹²³,Peacock Sharon J.¹²³

Affiliation:

1. Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand

2. Menzies School of Health Research, Charles Darwin University, Darwin, Northern Territory, Australia

3. Center for Clinical Vaccinology and Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Churchill Hospital, Oxford, United Kingdom

Abstract

ABSTRACT Five enzyme-linked immunosorbent assays developed to detect antibodies to different Burkholderia pseudomallei antigen preparations were evaluated as diagnostic tests for melioidosis in northeast Thailand. The highest diagnostic indices were observed for an affinity-purified antigen (sensitivity, 82%; specificity, 72%) and crude B. pseudomallei antigen (sensitivity, 81%; specificity, 70%), an improvement over the indirect hemagglutination assay (sensitivity, 73%; specificity, 64%).

Publisher

American Society for Microbiology

Subject

Microbiology (medical),Clinical Biochemistry,Immunology,Immunology and Allergy

Link

https://journals.asm.org/doi/pdf/10.1128/CVI.00289-06

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