Antibiotic Disc Susceptibility Tests for Rapid Presumptive Identification of Gram-Negative Anaerobic Bacilli

Author:

Sutter Vera L.12,Finegold Sydney M.12

Affiliation:

1. Anaerobic Bacteriology Laboratory and Department of Medicine, Wadsworth General Hospital, Veterans Administration, Los Angeles, California 90073

2. Department of Medicine, University of California at Los Angeles School of Medicine, Los Angeles, California 90024

Abstract

A method for identification of gram-negative anaerobic bacilli is described. Based on differences in susceptibility to paper discs containing 10 μg of colistin, 60 μg of erythromycin, 1,000 μg of kanamycin, 1,000 μg of neomycin, 2 units of penicillin, and 15 μg of rifampin, these bacteria may be placed into five groups. Other tests such as colony morphology, production of pigment, growth in bile, esculin hydrolysis, and reaction on egg yolk-agar may be used for further identification. The susceptibility tests are rapid and simple to perform and are helpful in characterizing gram-negative anaerobic bacilli. They are not intended for use in predicting clinical effectiveness of the drugs utilized.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference10 articles.

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4. Dowell V. R. Jr. and T. M. Hawkins. 1968. Laboratory methods in anaerobic bacteriology. U.S. Public Health Serv. Publ. No. 1803.

5. Antibiotic susceptibility patterns as aids in classification and characterization of gram-negative anaerobic bacilli;Finegold S. M.;J. Bacteriol.,1967

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