Potential Application of Nonstructural Protein NS1 Serotype-Specific Immunoglobulin G Enzyme-Linked Immunosorbent Assay in the Seroepidemiologic Study of Dengue Virus Infection: Correlation of Results with Those of the Plaque Reduction Neutralization Test-Reference-Cited by-同舟云学术

Potential Application of Nonstructural Protein NS1 Serotype-Specific Immunoglobulin G Enzyme-Linked Immunosorbent Assay in the Seroepidemiologic Study of Dengue Virus Infection: Correlation of Results with Those of the Plaque Reduction Neutralization Test

Published:2002-05 Issue:5 Volume:40 Page:1840-1844
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Shu Pei-Yun¹,Chen Li-Kuang²,Chang Shu-Fen¹,Yueh Yi-Yun¹,Chow Ling¹,Chien Li-Jung¹,Chin Chuan³,Yang Hui-Hua²,Lin Ting-Hsiang¹,Huang Jyh-Hsiung¹

Affiliation:

1. Division of Research and Diagnosis, Center for Disease Control, Department of Health

2. Department of Emergency Medicine, Medical Center, Tzu Chi University, Hualien, Taiwan, Republic of China

3. Co-Wealth Medical Science and Biotechnology, Taipei

Abstract

ABSTRACT An NS1 serotype-specific indirect enzyme-linked immunosorbent assay (ELISA) was developed to differentiate primary and secondary dengue virus infections and serotypes of primary dengue virus infection. For this report, we carried out retrospective seroepidemiologic studies on serum samples collected from residents of Liuchiu Hsiang, Pingtung County, an isolated island in southern Taiwan during 1997-1998. The results demonstrated that good correlation existed between dengue virus NS1 serotype-specific immunoglobulin G (IgG) ELISA and dengue virus plaque reduction neutralization test (PRNT). Our data suggested that NS1 serotype-specific IgG ELISA could replace PRNT for seroepidemiologic studies to differentiate Japanese encephalitis and dengue virus infections and for dengue virus serotyping.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.5.1840-1844.2002

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