Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs-Reference-Cited by-同舟云学术

Rapid PCR-Based Identification of Methicillin-Resistant Staphylococcus aureus from Screening Swabs

Published:2002-05 Issue:5 Volume:40 Page:1821-1823
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Jonas D.¹,Speck M.¹,Daschner F. D.¹,Grundmann H.²

Affiliation:

1. National Reference Center of Hygiene, Institute of Environmental Medicine and Hospital Epidemiology, University Hospital Freiburg, 79106 Freiburg, Germany

2. Department of Microbiology, University Hospital, Queens Medical Centre, Nottingham NG7 2UH, United Kingdom

Abstract

ABSTRACT A PCR identification of methicillin-resistant Staphylococcus aureus (MRSA), obviating the need for subculture on agar media, was investigated. The combination of MRSA detection by mecA femB PCR with prior enrichment in selective broth was tested for 439 swabs. PCR identified 36 MRSA-positive samples, in concordance with conventional methods.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.40.5.1821-1823.2002

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