Affiliation:
1. Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada K7L 3N6
Abstract
ABSTRACT
The baculovirus protein P143 is essential for viral DNA replication in vivo, likely as a DNA helicase. We have demonstrated that another viral protein, LEF-3, first described as a single-stranded DNA binding protein, is required for transporting P143 into the nuclei of insect cells. Both of these proteins, along with several other early viral proteins, are also essential for DNA replication in transient assays. We now describe the identification, nucleotide sequences, and transcription patterns of the
Choristoneura fumiferana
nucleopolyhedrovirus (Cf
M
NPV) homologues of
p143
and
lef-3
and demonstrate that Cf
M
NPV LEF-3 is also responsible for P143 localization to the nucleus. We predicted that the interaction between P143 and LEF-3 might be critical for cross-species complementation of DNA replication. Support for this hypothesis was generated by substitution of heterologous P143 and LEF-3 between two different baculovirus species,
Autographa californica
nucleopolyhedrovirus and Cf
M
NPV, in transient DNA replication assays. The results suggest that the P143-LEF-3 complex is an important baculovirus replication factor.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
23 articles.
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