Detection of Human Immunodeficiency Virus Type 1 Nef and CD4 Physical Interaction in Living Human Cells by Using Bioluminescence Resonance Energy Transfer-Reference-Cited by-同舟云学术

Detection of Human Immunodeficiency Virus Type 1 Nef and CD4 Physical Interaction in Living Human Cells by Using Bioluminescence Resonance Energy Transfer

Published:2005-07 Issue:13 Volume:79 Page:8629-8636
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Cluet David¹,Bertsch Christophe,Beyer Christian¹,Gloeckler Liliane¹,Erhardt Mathieu¹,Gut Jean-Pierre¹,Galzi Jean-Luc²,Aubertin Anne-Marie¹

Affiliation:

1. INSERM-ULP U544, Université Louis Pasteur, Institut de Virologie 3 Rue Koeberlé, 67000 Strasbourg, France

2. CNRS UPR 9050, Département Récepteurs et Protéines membranaires, ESBS, Boulevard Sébastien Brandt, 67400 Illkirch, France

Abstract

ABSTRACT CD4 down-regulation by human immunodeficiency virus type 1 (HIV-1) Nef protein is a key function for virus virulence. This activity may be mediated by a direct Nef-CD4 interaction. We investigated the formation, in situ, of such a complex between proteins using bioluminescence resonance energy transfer technology and coimmunoprecipitations. Our data clearly demonstrate that Nef and CD4 interact in intact human cells. Moreover, our results clearly indicate that the dileucine motif of the CD4 cytoplasmic domain, critical for the Nef-induced CD4 down-regulation, is not implicated in the Nef/CD4 complex formation in the cellular context.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.79.13.8629-8636.2005

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