Affiliation:
1. Institut für Medizinische Mikrobiologie und Immunologie, Universität Bonn, Bonn, Germany
Abstract
ABSTRACT
Genome replication of herpes simplex viruses (HSV) in cultured cells is thought to be started by the action of the virus-encoded origin-binding protein (OBP). In experiments using two HSV-1 mutants with temperature-sensitive lesions in the helicase domain of OBP, we demonstrated that this function is essential during the first 6 hours of the lytic cycle. Once DNA synthesis has started, this function is no longer required, suggesting that origin-driven initiation of viral DNA replication is a single event rather than a continuous process.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
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