Author:
DesGroseillers L,Villemur R,Jolicoeur P
Abstract
The Gross passage A murine leukemia virus (MuLV) is a highly leukemogenic, ecotropic fibrotropic retrovirus. Its genome is similar to that of other nonleukemogenic ecotropic fibrotropic MuLVs but differs at the 3' end and in the long terminal repeat. To determine whether these modifications were related to its leukemogenic potential, we constructed a viral DNA recombinant in vitro with cloned infectious DNA from this highly leukemogenic Gross passage A MuLV and from a weakly leukemogenic endogenous BALB/c B-tropic MuLV. Infectious viruses, recovered after microinjection of murine cells with recombinant DNA, were injected into newborn mice. We show here that the Gross passage A 1.35-kilobase-pair KpnI fragment (harboring part of gp70, all of p15E, and the long terminal repeat) is sufficient to confer a high leukemogenic potential to this recombinant.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Reference39 articles.
1. Bishop J. M. and H. E. Varmus. 1982. Functions and origins of retroviral transforming genes p. 999-1108. In R. A. Weiss et al. (ed.) RNA tumor viruses molecular biology of tumor viruses. Cold Spring Harbor Laboratory Cold Spring Harbor N.Y.
2. Most sequence differences between the genomes of the AKV virus and a leukemogenic Gross A virus passaged in vitro are located near the 3' terminus;Buchhagen D. L.;Proc. Natl. Acad. Sci. U.S.A.,1980
3. Leukemogenic activity of murine type C viruses after long-term passage in vitro;Buchhagen D. L.;Int. J. Cancer,1976
4. Cellular origin and role of mink cell focus-forming viruses in murine thymic Iymphomas;Chattopadhyay S. K.;Nature (London),1982
5. Lymphomagenicity of recombinant mink cell focus-inducing murine leukemia viruses;Cloyd M. W.;J. Exp. Med.,1980
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