Temporal Profiling of the Coding and Noncoding Murine Cytomegalovirus Transcriptomes-Reference-Cited by-同舟云学术

Temporal Profiling of the Coding and Noncoding Murine Cytomegalovirus Transcriptomes

Published:2011-06-15 Issue:12 Volume:85 Page:6065-6076
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Lacaze Paul¹,Forster Thorsten¹,Ross Alan¹,Kerr Lorraine E.²,Salvo-Chirnside Eliane²,Lisnic Vanda Juranic³,López-Campos Guillermo H.⁴,García-Ramírez José J.⁵,Messerle Martin⁶,Trgovcich Joanne⁷,Angulo Ana⁸,Ghazal Peter¹²

Affiliation:

1. Division of Pathway Medicine, The University of Edinburgh, The Chancellor's Building, College of Medicine, 49 Little France Crescent, Edinburgh, United Kingdom

2. Centre for Systems Biology at Edinburgh, The University of Edinburgh, Darwin Building, King's Buildings Campus, Mayfield Road, Edinburgh, United Kingdom

3. Department of Histology and Embryology, Faculty of Medicine, Rijeka University, Croatia

4. Área de Bioinformática y Salud Pública, Instituto de Salud Carlos III, 28220 Madrid, Spain

5. Department of Inorganic and Organic Chemistry and Biochemistry, Medical School, Regional Center for Biomedical Research, University of Castilla—La Mancha, Avenida de Almansa 14, 02006 Albacete, Spain

6. Department of Virology, Hannover Medical School, Hannover, Germany

7. Department of Pathology, The Ohio State University, Columbus, Ohio 43210

8. Institut d'Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Barcelona, Spain

Abstract

ABSTRACT The global transcriptional program of murine cytomegalovirus (MCMV), involving coding, noncoding, and antisense transcription, remains unknown. Here we report an oligonucleotide custom microarray platform capable of measuring both coding and noncoding transcription on a genome-wide scale. By profiling MCMV wild-type and immediate-early mutant strains in fibroblasts, we found rapid activation of the transcriptome by 6.5 h postinfection, with absolute dependency on ie3 , but not ie1 or ie2 , for genomic programming of viral gene expression. Evidence is also presented to show, for the first time, genome-wide noncoding and bidirectional transcription at late stages of MCMV infection.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.02341-10

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