Affiliation:
1. Max-Volmer-Institut für Biophysikalische Chemie und Biochemie, Fachgebiet Biochemie und Molekulare Biologie, Technische Universität Berlin, D-10587 Berlin, Germany
Abstract
ABSTRACT
A gene (
estA
) encoding a 42-kDa cell-bound esterase, EstA, was found to be located 75 bp upstream of the cyclophilin A gene (
cypA
) of
Streptomyces chrysomallus
. Western blot analysis revealed the presence of EstA (42 kDa) in cell extracts of
S. chrysomallus
X2 and
Streptomyces lividans
. EstA specifically hydrolyzes short-chain
p
-nitrophenyl esters. EstA formation starts at the end of growth phase, and its activity level remains constant throughout stationary phase. Expression of
estA
from the melanin (
mel
) promoter in plasmid pIJ702 led to a substantial increase of total esterase activity in streptomycetes.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
26 articles.
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