Value of the Chlorhexidine Decontamination Method for Recovery of Nontuberculous Mycobacteria from Sputum Samples of Patients with Cystic Fibrosis

Author:

Ferroni Agnès1,Vu-Thien Hoang2,Lanotte Philippe3,Le Bourgeois Muriel4,Sermet-Gaudelus Isabelle5,Fauroux Brigitte6,Marchand Sophie7,Varaigne Françoise8,Berche Patrick1,Gaillard Jean-Louis9,Offredo Catherine1

Affiliation:

1. Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Assistance Publique—Hôpitaux de Paris (AP-HP), Paris

2. Laboratoire de Microbiologie, Hôpital Trousseau, AP-HP, Paris

3. Laboratoire de Microbiologie, Hôpital Bretonneau, Tours

4. Service de Pneumologie-Allergologie Pédiatrique

5. Service de Pédiatrie Générale, Hôpital Necker-Enfants Malades, AP-HP, Paris

6. Service de Pneumologie Pédiatrique, Hôpital Trousseau, AP-HP, Paris

7. Service de Pédiatrie, Hôpital Pédiatrique Gatien de Clocheville, Tours

8. Service de Pneumologie, Hôpital Bretonneau, Tours

9. Laboratoire de Microbiologie, Hôpital Raymond Poincaré, AP-HP, Garches, France

Abstract

ABSTRACT The chlorhexidine method was compared to the N -acetyl- l -cysteine-NaOH-oxalic acid decontamination method currently recommended for the recovery of nontuberculous mycobacteria (NTM) from patients with cystic fibrosis. Sputum samples ( n = 827) treated with chlorhexidine yielded twice as many NTM-positive cultures as those treated by the reference method (54 [6.50%] versus 27 [3.25%]; P < 0.0001) despite a higher contamination rate (20% versus 14.2%; P = 0.0017).

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

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