Affiliation:
1. Laboratoire de Chimie Bactérienne—Centre National de la Recherche Scientifique, 31, Chemin Joseph-Aiguier, 13-Marseille (9e), France
Abstract
A method for the preparation of protoplasts from
Candida tropicalis
cultivated on
n
-tetradecane is described. This essentially consists of replacing the mannitol-sorbitol solution of the classical helicase technique by 1
m
magnesium sulfate and lowering the
p
H to 4.1 during incubation in the presence of helicase. The protoplasts thus prepared behave like intact cells and are capable of consuming oxygen in the presence of
n
-tetradecane,
n
-decane, 1-decanol, and glucose.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference7 articles.
1. Production of protoplast-like structure from various species of fungi;Aguirre M. J. R.;Nature,1962
2. Localization of the site of fixation of the inducer penicillin in Bacillus cereus;Duerksen J. D.;Biochim. Biophys. Acta,1964
3. A method of isolating protoplasts from yeast;Eddy A. A.;Nature,1957
4. Production of yeast and mold protoplasts by treatment with the strepzyme of Micromonospora sp;Gascon S.;Can. J. Microbiol.,1965
5. Oxidative phosphorylation in yeast. I. Isolation and properties of phosphorylating mitochondria from stationary phase cells;Kovac L.;Biochim. Biophys. Acta,1968
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