Author:
Cooper Moogega,La Duc Myron T.,Probst Alexander,Vaishampayan Parag,Stam Christina,Benardini James N.,Piceno Yvette M.,Andersen Gary L.,Venkateswaran Kasthuri
Abstract
ABSTRACTA bacterial spore assay and a molecular DNA microarray method were compared for their ability to assess relative cleanliness in the context of bacterial abundance and diversity on spacecraft surfaces. Colony counts derived from the NASA standard spore assay were extremely low for spacecraft surfaces. However, the PhyloChip generation 3 (G3) DNA microarray resolved the genetic signatures of a highly diverse suite of microorganisms in the very same sample set. Samples completely devoid of cultivable spores were shown to harbor the DNA of more than 100 distinct microbial phylotypes. Furthermore, samples with higher numbers of cultivable spores did not necessarily give rise to a greater microbial diversity upon analysis with the DNA microarray. The findings of this study clearly demonstrated that there is not a statistically significant correlation between the cultivable spore counts obtained from a sample and the degree of bacterial diversity present. Based on these results, it can be stated that validated state-of-the-art molecular techniques, such as DNA microarrays, can be utilized in parallel with classical culture-based methods to further describe the cleanliness of spacecraft surfaces.
Publisher
American Society for Microbiology
Subject
Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology
Reference36 articles.
1. Phylogenetic identification and in situ detection of individual microbial cells without cultivation;Amann;Microbiol. Rev.,1995
2. ASTM E2362-09. Standard practice for evaluation of pre-saturated or impregnated towelettes for hard surface disinfection;ASTM;ASTM book of standards,2009
3. Urban aerosols harbor diverse and dynamic bacterial populations;Brodie;Proc. Natl. Acad. Sci. U. S. A.,2007
4. Environmental genomics reveals a single-species ecosystem deep within Earth;Chivian;Science,2008
Cited by
28 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献