Affiliation:
1. Department of Pharmacology and Toxicology, Medical School, Hannover, Federal Republic of Germany.
Abstract
Development of Chlamydia trachomatis (L2/434/Bu) in HEp-2 cells was inhibited by treatment of the cells with recombinant human alpha tumor necrosis factor (TNF). In the infected cultures that were treated with TNF, high concentrations of prostaglandin E2(PGE2) were detected, exceeding by far the concentrations found in TNF-treated but uninfected cells or in infected cells that were not treated with TNF. PGE2 levels increased gradually for 2 days after infection. Raising the tryptophan concentration in the culture medium, which reversed the inhibition of chlamydial replication by TNF, also blocked the increase in PGE2 formation. However, neutralizing antibodies to beta interferon, which also interfered with the antichlamydial effect of TNF, did not decrease PGE2 formation. Excessive formation of PGE2 by cells infected with chlamydiae and treated by TNF might be related to some of the complications associated with chlamydial infection.
Publisher
American Society for Microbiology
Subject
Infectious Diseases,Immunology,Microbiology,Parasitology
Reference29 articles.
1. Aitken I. D. 1988. Animal chlamydial infection p. 11-13. In P. A. Csango M. La Placa J. Orfila I. Sarov and M. Ward (ed.) Proceedings of the European Society for Chlamydia Research. Societa Editrice Esculatio Bologna Italy.
2. Growing tumors induce hypersensitivity to endotoxin and tumor necrosis factor;Bartholeyns J.;Infect. Immun.,1987
3. Suppression of IL-1 bioactivity in synovial cell cultures by polyclonal anti-TNF antibody;Brennan F. M.;Lymphokine Res.,1988
4. Induction of tryptophan catabolism is the mechanism for gamma-interferon-mediated inhibition of intracellular Chlamydia psittaci replication in T24 cells;Byrne G. I.;Infect. Immun.,1986
5. Possible roles of tumor necrosis factor in the pathology of malaria;Clark I. A.;Am. J. Pathol.,1987
Cited by
40 articles.
订阅此论文施引文献
订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献