Rapid micro-carbohydrate test for confirmation of Neisseria gonorrhoeae

Author:

Yong D C,Prytula A

Abstract

A rapid carbohydrate utilization procedure for the confirmation of Neisseria gonorrhoeae and identification of other Neisseria species has been developed. This method utilizes both preformed enzymes, introduced in a heavy inoculum, and enzymes formed by the microorganisms as a result of growth in a small volume of super-enriched medium. Expected carbohydrate reactions were produced by 383 clinical isolates of neisseriae and were clearly visible within 4 h of incubation. The combined use of disposable glass tubes (6 by 50 mm) and microamounts of media (0.05 ml) make this method not only rapid, but also low in cost.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Reference27 articles.

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3. Identification of Neisseria meningitidis carbohydrate fermentation patterns in Mueller-Hinton broth;Beno D. W.;J. Bacteriol.,1968

4. Modification of the rapid fermentation test for Neisseria gonorrhoeae. Apple;Brown W. J.;Microbiol.,1974

5. A comparison of three fermentation methods for the confirmation of Neisseria gonorrhoeae;Brown W. J.;Health Lab. Sci.,1976

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