Development of High-Throughput Phenotyping of Metagenomic Clones from the Human Gut Microbiome for Modulation of Eukaryotic Cell Growth-Reference-Cited by-同舟云学术

Development of High-Throughput Phenotyping of Metagenomic Clones from the Human Gut Microbiome for Modulation of Eukaryotic Cell Growth

Published:2007-06 Issue:11 Volume:73 Page:3734-3737
ISSN:0099-2240
Container-title:Applied and Environmental Microbiology
language:en
Short-container-title:Appl Environ Microbiol

Author:

Gloux Karine¹,Leclerc Marion¹,Iliozer Harout¹,L'Haridon René²,Manichanh Chaysavanh¹,Corthier Gérard¹,Nalin Renaud³,Blottière Hervé M.¹,Doré Joël¹

Affiliation:

1. Unité d'Ecologie et Physiologie du Système Digestif

2. Unité de Virologie et Immunologie Moléculaires, Institut National de la Recherche Agronomique, Domaine de Vilvert, 78352 Jouy en Josas, France

3. LibraGen, Bat. Canal Biotech I, 3 rue des Satellites, 31400 Toulouse, France

Abstract

ABSTRACT Metagenomic libraries derived from human intestinal microbiota (20,725 clones) were screened for epithelial cell growth modulation. Modulatory clones belonging to the four phyla represented among the metagenomic libraries were identified (hit rate, 0.04 to 8.7% depending on the screening cutoff). Several candidate loci were identified by transposon mutagenesis and subcloning.

Publisher

American Society for Microbiology

Subject

Ecology,Applied Microbiology and Biotechnology,Food Science,Biotechnology

Link

https://journals.asm.org/doi/pdf/10.1128/AEM.02204-06

Reference32 articles.

1. Alam, M., T. Midtvedt, and A. Uribe. 1994. Differential cell kinetics in the ileum and colon of germfree rats. Scand. J. Gastroenterol.29:445-451.

2. Evolutionary Analyses of the Small Subunit of Glutamate Synthase: Gene Order Conservation, Gene Fusions, and Prokaryote-to- Eukaryote Lateral Gene Transfers

3. Backhed, F., H. Ding, T. Wang, L. V. Hooper, G. Y. Koh, A. Nagy, C. F. Semenkovich, and J. I. Gordon. 2004. The gut microbiota as an environmental factor that regulates fat storage. Proc. Natl. Acad. Sci. USA101:15718-15723.

4. Backhed, F., R. E. Ley, J. L. Sonnenburg, D. A. Peterson, and J. I. Gordon. 2005. Host-bacterial mutualism in the human intestine. Science307:1915-1920.

5. Blottiere, H. M., B. Buecher, J. P. Galmiche, and C. Cherbut. 2003. Molecular analysis of the effect of short-chain fatty acids on intestinal cell proliferation. Proc. Nutr. Soc.62:101-106.

Cited by 53 articles. 订阅此论文施引文献订阅此论文施引文献，注册后可以免费订阅5篇论文的施引文献，订阅后可以查看论文全部施引文献

1. An Insight into Functional Metagenomics: A High-Throughput Approach to Decipher Food–Microbiota–Host Interactions in the Human Gut;International Journal of Molecular Sciences;2023-12-18

2. New carbohydrate binding domains identified by phage display based functional metagenomic screens of human gut microbiota;Communications Biology;2023-04-05

3. The Microbiota and the Relationship with Colorectal Cancer: Surgical Complications—A Review;Gastrointestinal Disorders;2022-04-29

4. Metagenomic Bioprospecting of Uncultivable Microbial Flora in Soil Microbiome for Novel Enzymes;Geomicrobiology Journal;2021-12-26

5. Investigation of cold-active and mesophilic cellulases: opportunities awaited;Biomass Conversion and Biorefinery;2021-11-04