Affiliation:
1. The Johns Hopkins University School of Hygiene and Public Health, Baltimore, Maryland 21205
Abstract
A chemically defined medium (MI
c
) is described in which cells of
Haemophilus influenzae
Rd grow rapidly (generation time, 35 ± 5 min) and reach a stationary level of 10
10
cells/ml. Our strain of cells grown in this medium developed high levels of competence when transferred to another medium designed for that purpose. Conditions governing the total development of competence in this organism have now been defined.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
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