Correlation of Melting Temperature and Cesium Chloride Buoyant Density of Bacterial Deoxyribonucleic Acid-Reference-Cited by-同舟云学术

Correlation of Melting Temperature and Cesium Chloride Buoyant Density of Bacterial Deoxyribonucleic Acid

Published:1970-02 Issue:2 Volume:101 Page:333-338
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Mandel M.¹,Igambi Levi¹,Bergendahl Janet¹,Dodson M. L.¹,Scheltgen E.¹

Affiliation:

1. Section of Molecular Biology, The University of Texas M. D. Anderson Hospital and Tumor Institute at Houston, Houston, Texas 77025

Abstract

Samples of bacterial deoxyribonucleic acid from bacteria having guanine plus cytosine (GC) contents in the range of 27 to 72 moles per cent GC were analyzed by optical melting (T m ) and equilibrium buoyant density methods. The relation between these properties is shown to be linear. The relative value of 1.99 moles per cent GC per degree C change in T m is calculated, and a reference method for the calculation of GC contents relative to a standard is derived.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jb.101.2.333-338.1970

Reference17 articles.

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2. Deoxyribonucleic acid base composition of acetic acid bacteria;DeLey J.;J. Gen. Microbiol.,1963

3. Cation effects on the denaturation of DNA;Dove W. F.;J. Mol. Biol.,1962

4. Deoxyribonucleic acid base composition of the genus Lactobacillus;Gasser F.;J. Bacteriol.,1968

5. Salt effects on the denaturation of DNA;Gruenwedel D. W.;Biopolymers,1969

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