Affiliation:
1. State Key Laboratories for Agro-biotechnology and Ministry of Agriculture Key Laboratory of Soil Microbiology, College of Biological Sciences, China Agricultural University, Beijing, China
Abstract
ABSTRACT
The role of a
tetR
transcriptional regulatory gene (
SAV7471
) in avermectin production in the Gram-positive soil bacterium
Streptomyces avermitilis
was investigated by gene deletion, complementation, and overexpression experiments. Gene deletion of the
SAV7471
open reading frame resulted in avermectin overproduction. The deletion also resulted in overexpression of
SAV7472-SAV7473
transcripts, which encode a protein of unknown function and a flavoprotein possibly involved in pantothenate and coenzyme A (CoA) metabolism. EMSAs and footprinting assays showed that SAV7471 can bind to two palindromic sequences with high similarity in the intergenic region between
SAV7471
and
SAV7472
, a region that contains the apparent transcription start sites for each gene detected by rapid amplification of 5′ cDNA ends (5′-RACE). In addition to
SAV7472-SAV7473
, at least two genes (
SAV1104
and
SAV1258
) involved in CoA metabolism are negatively controlled by SAV7471. By negatively regulating the transcription of the target genes
SAV7472-SAV7473
and other genes involved in CoA metabolism, SAV7471 may affect cellular metabolic flux and may thereby indirectly regulate avermectin biosynthesis.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
35 articles.
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