Deep Sequencing Analysis of Individual HIV-1 Proviruses Reveals Frequent Asymmetric Long Terminal Repeats-Reference-Cited by-同舟云学术

Deep Sequencing Analysis of Individual HIV-1 Proviruses Reveals Frequent Asymmetric Long Terminal Repeats

Published:2022-07-13 Issue:13 Volume:96 Page:
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Joseph Kevin W.¹,Halvas Elias K.¹,Brandt Leah D.¹,Patro Sean C.²,Rausch Jason W.³,Chopra Abha⁴,Mallal Simon⁴⁵,Kearney Mary F.²,Coffin John M.⁶,Mellors John W.¹^ORCID

Affiliation:

1. University of Pittsburgh, Department of Medicine, Division of Infectious Diseases, Pittsburgh, Pennsylvania, USA

2. HIV Dynamics and Replication Program, National Cancer Institute, Frederick, Maryland, USA

3. Basic Research Laboratory, Center for Cancer Research, National Cancer Institute, Frederick, Maryland, USA

4. Institute for Immunology & Infectious Diseases, Murdoch University, Murdoch, Western Australia, Australia

5. University of Vanderbilt, Department of Medicine, Nashville, Tennessee, USA

6. Tufts University, Boston, Massachusetts, USA

Abstract

The integration of human immunodeficiency virus type 1 (HIV-1) into chromosomal DNA establishes the long-term persistence of HIV-1 as proviruses despite effective antiretroviral therapy (ART). Characterizing proviruses is difficult because of their rarity in individuals on long-term suppressive ART, their highly polymorphic sequences and genetic structures, and the need for efficient amplification and sequencing of the provirus and its integration site.

Funder

American Cancer Society

Bill and Melinda Gates Foundation

HHS | NIH | National Cancer Institute

HHS | NIH | National Institute of Allergy and Infectious Diseases

HHS | NIH | Office of AIDS Research

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/jvi.00122-22