Immunofluorescence Technique for the Detection of Salmonellae in Various Foods

Author:

Insalata N. F.1,Schulte S. J.1,Berman J. H.1

Affiliation:

1. General Foods Technical Center, Tarrytown, New York 10591

Abstract

Salmonella species have been detected in nine food varieties by use of fluorescent antibodies without false-positive or false-negative results. Test antisera were specially prepared, commercially available, conjugated polyvalent O globulin absorbed with cultures of Escherichia coli and Citrobacter freundii , and polyvalent phase II H globulin antibodies. Use of this technique permits a decrease of 24 hr in time normally required for Salmonella detection when compared with cultural Salmonella recovery methods.

Publisher

American Society for Microbiology

Subject

General Pharmacology, Toxicology and Pharmaceutics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,General Medicine

Reference15 articles.

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3. The demonstration of pneumococcal antigen in tissue by the use of fluorescent antibodies;COONS A. H.;J. Immunol.,1942

4. A rapid immunofluorescent technique for detecting salmonellae in raw meat;GEORGALA D. L.;J. Hyg.,1964

5. Further evaluation of a rapid immunofluorescent technique for detecting salmonellae in meat and poultry;GEORGALA D. L.;J. Appl. Bacteriol.,1965

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