Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication-Reference-Cited by-同舟云学术

Amphipathic Helix-Dependent Localization of NS5A Mediates Hepatitis C Virus RNA Replication

Published:2003-05-15 Issue:10 Volume:77 Page:6055-6061
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Elazar Menashe¹,Cheong Kwang Ho¹²,Liu Ping¹,Greenberg Harry B.¹²,Rice Charles M.³,Glenn Jeffrey S.¹²

Affiliation:

1. Division of Gastroenterology and Hepatology, Stanford University School of Medicine

2. Department of Microbiology and Immunology, Veterans Administration Medical Center, Palo Alto, California

3. Center for the Study of Hepatitis C, Rockefeller University, New York, New York

Abstract

ABSTRACT We identified an N-terminal amphipathic helix (AH) in one of hepatitis C virus (HCV)’s nonstructural proteins, NS5A. This AH is necessary and sufficient for membrane localization and is conserved across isolates. Genetically disrupting the AH impairs HCV replication. Moreover, an AH peptide-mimic inhibits the membrane association of NS5A in a dose-dependent manner. These results have exciting implications for the HCV life cycle and novel antiviral strategies.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.77.10.6055-6061.2003

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