Hepatitis C Virus-Like Particle Budding: Role of the Core Protein and Importance of Its Asp 111-Reference-Cited by-同舟云学术

Hepatitis C Virus-Like Particle Budding: Role of the Core Protein and Importance of Its Asp 111

Published:2003-09-15 Issue:18 Volume:77 Page:10131-10138
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Blanchard Emmanuelle¹,Hourioux Christophe¹²,Brand Denys¹,Ait-Goughoulte Malika¹,Moreau Alain¹,Trassard Sylvie²,Sizaret Pierre-Yves²,Dubois Frederic¹³,Roingeard Philippe¹²

Affiliation:

1. Laboratoire de Virologie

2. Laboratoire de Biologie Cellulaire, Faculté de Médecine et Centre Hospitalier Universitaire, Tours

3. Institut Inter Régional de la Santé, La Riche, France

Abstract

ABSTRACT In the absence of a hepatitis C virus (HCV) culture system, the use of a Semliki Forest virus replicon expressing genes encoding HCV structural proteins that assemble into HCV-like particles provides an opportunity to study HCV morphogenesis. Using this system, we showed that the HCV core protein constitutes the budding apparatus of the virus and that its targeting to the endoplasmic reticulum by means of the signal sequence of E1 protein is essential for budding. In addition, the aspartic acid at position 111 in the HCV core protein sequence was found to be crucial for virus assembly, demonstrating the usefulness of this system for mapping amino acids critical to HCV morphogenesis.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.77.18.10131-10138.2003

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