Affiliation:
1. Sexually Transmitted and Blood Borne Virus Laboratory, Central Public Health Laboratory, London NW9 5HT, United Kingdom
2. Cercopan Conservation Centre, Calabar, Nigeria
Abstract
ABSTRACT
A novel simian immunodeficiency virus (SIV) sequence has been recovered from RNA extracted from the serum of a mona monkey (
Cercopithecus mona
) wild born in Nigeria. The sequence was obtained by using novel generic (degenerate) PCR primers and spans from two-thirds into the
gag
gene to the 3′ poly(A) tail of the SIVmonNG1 RNA genome. Analysis of the open reading frames revealed that the SIVmonNG1 genome codes for a Vpu protein, in addition to Gag, Pol, Vif, Vpr, Tat, Rev, Env, and Nef proteins. Previously, only lentiviruses infecting humans (human immunodeficiency virus type 1 [HIV-1]) and chimpanzees (SIVcpz) were known to have a
vpu
gene; more recently, this has also been found in SIVgsn from
Cercopithecus nictitans
. Overall, SIVmonNG1 most closely resembles SIVgsn: the
env
gene sequence groups with HIV-1/SIVcpz
env
sequences, whereas the
pol
gene sequence clusters closely with the
pol
sequence of SIVsyk from
Cercopithecus albogaris
. By bootscanning and similarity plotting, the first half of
pol
resembles SIVsyk, whereas the latter part is closer to SIVcol from
Colobus guereza
. The similarities between the complex mosaic genomes of SIVmonNG1 and SIVgsn are consistent with a shared or common lineage. These data further highlight the intricate nature of the relationships between the SIVs from different primate species and will be helpful for unraveling these associations.
Publisher
American Society for Microbiology
Subject
Virology,Insect Science,Immunology,Microbiology
Cited by
41 articles.
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