Identification of White Spot Syndrome Virus Latency-Related Genes in Specific-Pathogen-Free Shrimps by Use of a Microarray-Reference-Cited by-同舟云学术

Identification of White Spot Syndrome Virus Latency-Related Genes in Specific-Pathogen-Free Shrimps by Use of a Microarray

Published:2003-09-15 Issue:18 Volume:77 Page:10162-10167
ISSN:0022-538X
Container-title:Journal of Virology
language:en
Short-container-title:J Virol

Author:

Khadijah Siti¹,Neo Soek Ying²,Hossain M. S.¹,Miller Lance D.²,Mathavan S.²,Kwang Jimmy¹

Affiliation:

1. Animal Health Biotechnology, Temasek Life Sciences Laboratory, The National University of Singapore, Singapore 117604

2. Genome Institute of Singapore, Singapore 117528

Abstract

ABSTRACT To investigate whether specific-pathogen-free (SPF) shrimps are asymptomatic carriers of white spot syndrome virus (WSSV), we used a WSSV-specific DNA microarray to measure WSSV gene expression in SPF and WSSV-infected shrimps. Three WSSV genes were found to be relatively highly expressed in SPF shrimps. Reverse transcription-PCR using nested primers as well as real-time detection confirmed that these genes have no detectable counterparts in GenBank; structural analysis of the putative proteins revealed helix-loop-helix and leucine zipper motifs. Viral sequences could be PCR amplified from genomic DNA of SPF shrimp, further supporting the suggestion that these shrimps are asymptomatic carriers.

Publisher

American Society for Microbiology

Subject

Virology,Insect Science,Immunology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JVI.77.18.10162-10167.2003

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