Affiliation:
1. Laboratory for Microbiology, Faculty of Sciences, State University, Ghent, Belgium
Abstract
De Ley
, J. (State University, Ghent, Belgium),
and S. Friedman
. Similarity of
Xanthomonas
and
Pseudomonas
deoxyribonucleic acid. J. Bacteriol.
89:
1306–1309. 1965.—Pronounced deoxyribonucleic acid (DNA) hybridization was detected with the DNA-agar method within the group of
Xanthomonas
and between
Xanthomonas
and
Pseudomonas
. Sheared, denatured C
14
-DNA from
X. pelargonii
hybridized 80 to 100% with high molecular weight, denatured agar-bound DNA from eight different
Xanthomonas
nomenspecies. The near identity of the DNA in these nine strains favors their inclusion in one genetic species, for which the name
Xanthomonas campestris
is proposed. In addition, 60 to 80% of the
Xanthomonas
DNA hybridized with DNA from several
Pseudomonas
strains. These results are evidence of the close genetic relationship between the two genera.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference2 articles.
1. BOLTON , E. T. , AND B . J. MCCARTHY. 1962 . A general method for the isolation of RNA complementary to DNA. Proc. Natl. Acad. Sci. U.S. 48:1390-1397.
2. COLWELL , R. R. , AND M . MANDEL. 1964 . Adan- 1309
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