Affiliation:
1. Department of Bacteriology, University of Wisconsin, Madison, Wisconsin
Abstract
Vary
, J. C. (University of Wisconsin, Madison),
and H. O. Halvorson
. Kinetics or germination of
Bacillus
spores. J. Bacteriol.
89:
1340–1347. 1965.—The kinetics of germination of
Bacillus cereus
strain T spores was accurately described by McCormick. To study the mechanism of germination, it is necessary to correlate the characteristic changes in a population of germinating spores with the behavior of the individual spores in the same population. Two microscopic events are apparent during germination: microlag, the time interval between the addition of
l
-alanine to heat-activated spores and the beginning of loss in refractility, and microgermination time, the time for the actual change in refractility to occur. The frequency distributions of both events are skewed, and appear to be independent. The effects of
l
-alanine concentration, heat activation, and temperature of germination on three parameters, microlag, microgermination, and per cent germination, were microscopically studied. The data are discussed in relation to the mechanism of germination, and a correlation between microlag and microgermination times with the constants of McCormick's equation has been suggested.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference13 articles.
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