Affiliation:
1. Department of Microbiology and Public Health, Michigan State University, East Lansing, Michigan 48823
Abstract
Cysts of
Azotobacter vinelandii
were ruptured with 3.0 m
m
ethylenediaminetetraacetate in 0.05
m
tris(hydroxymethyl)aminomethane buffer (
p
H 7.8) and fractionated by differential centrifugation in density gradients of sucrose or Ficoll (polysucrose) at 10,000 ×
g
for 2 hr at 4 C. The average densities of the cyst exine, the cyst central body, the vegetative cell, and the intact cyst were found to be 1.13, 1.26, 1.28, and 1.31 g/cm
3
in sucrose, and 1.08, 1.12, 1.13, and 1.17 g/cm
3
in Ficoll, respectively. These data were utilized to devise procedures for the isolation of cyst components. Cyst exine appeared as a multilayered structure in ultrathin sections. This same sheetlike structure was seen when exines were subjected to detergent treatment, metal-shadowed, and examined in an electron microscope. The exine was lysed by trypsin and was resistant to lysozyme, indicating that protein may be the principal structural material of the outer cyst coat.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Cited by
19 articles.
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