Affiliation:
1. Laboratory of Chemical Biology, National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland 20014
2. Laboratory of Molecular Biology, National Institute of Arthritis and Metabolic Diseases, Bethesda, Maryland 20014
Abstract
A kinetic study of induction of the enzymes of the lactose operon was carried out under conditions known to affect the kinetics of derepression of the enzymes of the histidine operon. The results show that the lactose system is similar to the histidine system in its responsiveness to conditions thought to affect the formylating capacity of the cell. This was demonstrated in the following ways: (i) trimethoprim, which is known to reduce the formylating capacity of the cell, gives rise to a relatively long interval between the times of induction of β-galactosidase and transacetylase; (ii) under conditions in which the histidine operon is derepressed, chloramphenicol causes a prolongation of the interval between the times of induction of the two enzymes, and this prolongation is reversed by adenine, methionine, and serine, compounds known to enrich the one-carbon pool of the cell; and (iii) 4-amino-5-imidazolcarboxamide ribonucleoside, a compound which may act as a drain for formyl groups, reverses the effect of the latter compounds. The finding that the interval between the times of induction of the two enzymes is shortened under conditions expected to maintain a relatively high intracellular fo rmylating capacity suggests that under certain conditions translation of the polycistronic messenger ribonucleic acid of the lactose operon may be initiated at more than one site or may proceed more rapidly from the operator end.
Publisher
American Society for Microbiology
Subject
Molecular Biology,Microbiology
Reference27 articles.
1. On the release of the formyl group from nascent protein;Adams J. M.;J. Mol. Biol.,1968
2. N-formylmethionylsRNA as the initiator of protein synthesis;Adams J. M.;Proc. Nat. Acad. Sci. U.S.A.,1966
3. Sequential transcription of the genes of the lactose operon and its regulation by protein synthesis;Alpers D. H.;J. Biol. Chem.,1966
4. Ames B. N. R. F. Goldberger P. E. Hartman R. G. Martin and J. R. Roth. 1967. The histidine operon p. 272-287. In V. V. Konigsberger and L. Bosch (ed.) Regulation of nucleic acid and protein biosynthesis. Elsevier Publishing Company Amsterdam.
5. The histidine operon;Ames B. N.;Cold Spring Harbor Symp. Quant. Biol.,1963
Cited by
6 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献