Genomic Sequencing Reveals Regulatory Mutations and Recombinational Events in the Widely Used MC4100 Lineage of Escherichia coli K-12

Author:

Ferenci Thomas1,Zhou Zhemin2,Betteridge Thu1,Ren Yan2,Liu Yu2,Feng Lu23,Reeves Peter R.1,Wang Lei234

Affiliation:

1. School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales, Australia

2. TEDA School of Biological Sciences and Biotechnology, Nankai University

3. Tianjin Research Center for Functional Genomics and Biochip, 23 Hongda Street, Tianjin Economic-Technological Development Area (TEDA), Tianjin 300457, China

4. Tianjin Key Laboratory of Microbial Functional Genomics, Nankai University, 23 Hongda Street, Tianjin Economic-Technological Development Area (TEDA), Tianjin 300457, China

Abstract

ABSTRACT The genome of an Escherichia coli MC4100 strain with a λ p lac Mu50 fusion revealed numerous regulatory differences from MG1655, including one that arose during laboratory storage. The 194 mutational differences between MC4100(MuLac) and other K-12 sequences were mostly allocated to specific lineages, indicating the considerable mutational divergence between K-12 strains.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Reference35 articles.

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2. Becker, G., E. Klauck, and R. Hengge-Aronis. 2000. The response regulator RssB, a recognition factor for σS proteolysis in Escherichia coli, can act like an anti-σS factor. Mol. Microbiol. 35 : 657-666.

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