Cloning and Expression of Two Genes Coding for Sodium Pumps in the Salt-Tolerant Yeast Debaryomyces hansenii-Reference-Cited by-同舟云学术

Cloning and Expression of Two Genes Coding for Sodium Pumps in the Salt-Tolerant Yeast Debaryomyces hansenii

Published:2001-05-15 Issue:10 Volume:183 Page:3251-3255
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Almagro Anabel¹²,Prista Catarina³,Benito Begoña²,Loureiro-Dias Maria C.³,Ramos José¹

Affiliation:

1. Departamento de Microbiologı́a, Escuela Técnica Superior de Ingenieros Agrónomos, E-14071 Córdoba,1 and

2. Departamento de Biotecnologı́a, Escuela Técnica Superior de Ingenieros Agrónomos, Universidad Politécnica de Madrid, E-28040, Madrid,2 Spain, and

3. Departamento de Botânica e Engenharia Biológica, Instituto Superior de Agronomia, 1349-017 Lisbon, Portugal3

Abstract

ABSTRACT Two genes encoding Na + -ATPases from Debaryomyces hansenii were cloned and sequenced. The genes, designated ENA1 from D. hansenii ( DhENA1 ) and DhENA2 , exhibited high homology with the corresponding genes from Schwanniomyces occidentalis. DhENA1 was expressed in the presence of high Na + concentrations, while the expression of DhENA2 also required high pH. A mutant of Saccharomyces cerevisiae lacking the Na + efflux systems and sensitive to Na + , when transformed with DhENA1 or DhENA2 , recovered Na + tolerance and also the ability to extrude Na + .

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.183.10.3251-3255.2001

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