Dehalogenation of Dichloromethane by Dichloromethane Dehalogenase/Glutathione S -Transferase Leads to Formation of DNA Adducts-Reference-Cited by-同舟云学术

Dehalogenation of Dichloromethane by Dichloromethane Dehalogenase/Glutathione S -Transferase Leads to Formation of DNA Adducts

Published:2001-09 Issue:17 Volume:183 Page:5209-5212
ISSN:0021-9193
Container-title:Journal of Bacteriology
language:en
Short-container-title:J Bacteriol

Author:

Kayser Martin F.¹,Vuilleumier Stéphane¹

Affiliation:

1. Institut für Mikrobiologie, ETH Zürich, CH-8092 Zürich, Switzerland

Abstract

ABSTRACT Formation of DNA adducts following conversion of dichloromethane by bacterial dichloromethane dehalogenase/glutathione S -transferase was demonstrated. Adducts included dichloromethane carbon and glutathione sulfur atoms. A reaction with DNA occurred preferentially at guanine bases. Increased DNA degradation in a polA mutant of Methylobacterium dichloromethanicum DM4 grown with dichloromethane confirmed the genotoxicity associated with dichloromethane degradation, suggesting an important role of DNA repair in the metabolism of halogenated, DNA-alkylating compounds by bacteria.

Publisher

American Society for Microbiology

Subject

Molecular Biology,Microbiology

Link

https://journals.asm.org/doi/pdf/10.1128/JB.183.17.5209-5212.2001

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