Detection of Cryptosporidium parvum in Horses: Thresholds of Acid-Fast Stain, Immunofluorescence Assay, and Flow Cytometry-Reference-Cited by-同舟云学术

Detection of Cryptosporidium parvum in Horses: Thresholds of Acid-Fast Stain, Immunofluorescence Assay, and Flow Cytometry

Published:1999-02 Issue:2 Volume:37 Page:457-460
ISSN:0095-1137
Container-title:Journal of Clinical Microbiology
language:en
Short-container-title:J Clin Microbiol

Author:

Cole D. J.¹,Snowden K.²,Cohen N. D.¹,Smith R.²

Affiliation:

1. Department of Large Animal Medicine and Surgery1 and

2. Department of Veterinary Pathobiology,2 College of Veterinary Medicine, Texas A&M University, College Station, Texas 77843-4475

Abstract

ABSTRACT Feces collected from three asymptomatic horses and seeded with Cryptosporidium parvum oocysts (10 1 to 10 6 /g of feces) were evaluated by acid-fast staining (AF), an immunofluorescent antibody (IFA) technique, and flow cytometry. The thresholds of detection were 5 × 10 5 oocysts/g of feces for the IFA and AF techniques and 5 × 10 4 oocysts/g for flow cytometry.

Publisher

American Society for Microbiology

Subject

Microbiology (medical)

Link

https://journals.asm.org/doi/pdf/10.1128/JCM.37.2.457-460.1999

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